Reaction Details | |||
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Cell Reactant: | Major Urinary Protein (MUP-1) | ||
Syringe Reactant: | BDBM12031 | ||
Meas. Tech.: | Isothermal Titration Calorimetry | ||
Entry Date: | 11/12/06 | ||
ΔG°: | -9.21±0.0300 (kcal/mole) | ||
pH: | 7.4±n/a | ||
Log10Kb: | 6.52± n/a | ||
Temperature: | 308±n/a (K) | ||
ΔH° : | n/a | ||
ΔHobs : | -11.5±0.210 (kcal/mole) | ||
Ionic Strength: | n/a | ||
not known | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | 1.01 | ||
ΔS° : | -0.0100±0 (kcal/mole-K) | ||
Comments: | n/a | ||
Citation | Bingham, RJ; Findlay, JB; Hsieh, SY; Kalverda, AP; Kjellberg, A; Perazzolo, C; Phillips, SE; Seshadri, K; Trinh, CH; Turnbull, WB; Bodenhausen, G; Homans, SW Thermodynamics of Binding of 2-Methoxy-3-isopropylpyrazine and 2-Methoxy-3-isobutylpyrazine to the Major Urinary Protein J Am Chem Soc126:1675-1681 (2004) Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
Major Urinary Protein (MUP-1) | |||
Source: | The MUP-1 gene was cloned and expressed in E. coli cells. | ||
Purity: | n/a | ||
Prep. Method: | MUP-I was purified using Ni-NTA resin. To remove endogenous bound ligands, an ethanol precipitation step was utilized involving addition of 2 volumes of absolute ethanol per volume of protein solution, followed by incubation at 4 °C for 2 h. After centrifugation at 5000g, the pellet was lyophilised, resuspended in PBS pH 7.4, and dialyzed overnight against the same buffer. | ||
Name: | Major Urinary Protein (MUP-1) | ||
Synonyms: | MUP1_MOUSE | Major urinary protein 1 precursor | Mup1 | ||
Type: | Other Protein Type | ||
Mol. Mass.: | 20640.17 | ||
Organism: | Mus musculus (mouse) | ||
Description: | n/a | ||
Residue: | 180 | ||
Sequence: |
| ||
BDBM12031 | |||
Source: | n/a | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name | BDBM12031 | ||
Synonyms: | 2-ISOBUTYL-3-METHOXYPYRAZINE | 2-methoxy-3-(2-methylpropyl)pyrazine | IBMP | ||
Type | Small organic molecule | ||
Emp. Form. | C9H14N2O | ||
Mol. Mass. | 166.2203 | ||
SMILES | COc1nccnc1CC(C)C | ||
Structure |
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