Reaction Details | |||
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Cell Reactant: | Major Urinary Protein (MUP-1) | ||
Syringe Reactant: | BDBM22603 | ||
Meas. Tech.: | Isothermal Titration Calorimetry | ||
Entry Date: | 06/15/08 | ||
ΔG°: | -5.53±0.0200 (kcal/mole) | ||
pH: | 7.4±n/a | ||
Log10Kb: | n/a | ||
Temperature: | 300±n/a (K) | ||
ΔH° : | n/a | ||
ΔHobs : | -9.81±0.770 (kcal/mole) | ||
Ionic Strength: | n/a | ||
no | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | 1.1 | ||
ΔS° : | -0.0100±0 (kcal/mole-K) | ||
Comments: | n/a | ||
Citation | Malham, R; Johnstone, S; Bingham, RJ; Barratt, E; Phillips, SE; Laughton, CA; Homans, SW Strong solute-solute dispersive interactions in a protein-ligand complex. J Am Chem Soc127:17061-7 (2005) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
Major Urinary Protein (MUP-1) | |||
Source: | The MUP-1 gene was cloned and expressed in E. coli cells. | ||
Purity: | n/a | ||
Prep. Method: | MUP-I was purified using Ni-NTA resin. To remove endogenous bound ligands, an ethanol precipitation step was utilized involving addition of 2 volumes of absolute ethanol per volume of protein solution, followed by incubation at 4 °C for 2 h. After centrifugation at 5000g, the pellet was lyophilised, resuspended in PBS pH 7.4, and dialyzed overnight against the same buffer. | ||
Name: | Major Urinary Protein (MUP-1) | ||
Synonyms: | MUP1_MOUSE | Major urinary protein 1 precursor | Mup1 | ||
Type: | Other Protein Type | ||
Mol. Mass.: | 20640.17 | ||
Organism: | Mus musculus (mouse) | ||
Description: | n/a | ||
Residue: | 180 | ||
Sequence: |
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BDBM22603 | |||
Source: | n/a | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name | BDBM22603 | ||
Synonyms: | 1-pentanol | Pentan-1-ol | n-pentanol | pentanol | ||
Type | Small organic molecule | ||
Emp. Form. | C5H12O | ||
Mol. Mass. | 88.1482 | ||
SMILES | CCCCCO | ||
Structure |
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