Reaction Details | |||
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Cell Reactant: | ferricytochrome c peroxidase (CcP) | ||
Syringe Reactant: | iso-1-cytochrome c (Cc) | ||
Meas. Tech.: | ITC | ||
Entry Date: | 12/15/00 | ||
ΔG°: | -8.14±0.300 (kcal/mole) | ||
pH: | 6±n/a | ||
Log10Kb: | n/a | ||
Temperature: | 298.15±n/a (K) | ||
ΔH° : | -3.92±0.100 (kcal/mole) | ||
ΔHobs : | -2.41± (kcal/mole) | ||
Ionic Strength: | n/a | ||
yes | |||
Protons Released: | 0.6 | ||
ΔCp : | -0.22±0.06 (kcal/mole) | ||
Stoich. Param.: | 1 | ||
ΔS° : | 0.0200±0 (kcal/mole-K) | ||
Comments: | test the effect of ionic strengh on binding | ||
Citation | Wang, X; Pielak, GJ Equilibrium thermodynamics of a physiologically-relevant heme-protein complex. Biochemistry38:16876-81 (1999) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
ferricytochrome c peroxidase (CcP) | |||
Source: | recombinant yeast CcP was expressed and purified from E.coli | ||
Purity: | n/a | ||
Prep. Method: | CcP and Cc were dialyzed (Slide -A_Lyzer membrane) against buffer in the same beaker for > 36 h with three buffer changes | ||
Name: | ferricytochrome c peroxidase (CcP) | ||
Synonyms: | n/a | ||
Type: | Enzyme | ||
Mol. Mass.: | 40387.06 | ||
Organism: | Saccharomyces cerevisiae | ||
Description: | A0A0D3U836 | ||
Residue: | 362 | ||
Sequence: |
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iso-1-cytochrome c (Cc) | |||
Source: | purified from yeast | ||
Purity: | n/a | ||
Prep. Method: | CcP and Cc were dialyzed (Slide -A_Lyzer membrane) against buffer in the same beaker for > 36 h with three buffer changes | ||
Name: | iso-1-cytochrome c (Cc) | ||
Synonyms: | n/a | ||
Type: | Protein | ||
Mol. Mass.: | 12191.93 | ||
Organism: | Saccharomyces cerevisiae | ||
Description: | A0A0C6AZG8 | ||
Residue: | 109 | ||
Sequence: |
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