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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | Cytochrome P450 Reductase (CPR) | ||
| Syringe Reactant: | BDBM11939 | ||
| Meas. Tech.: | Isothermal Titration Calorimetry | ||
| Entry Date: | 11/07/06 | ||
| ΔG°: | -8.30± (kcal/mole) | ||
| pH: | 7±n/a | ||
| Log10Kb: | 6.11± 4.70 | ||
| Temperature: | 298.15±n/a (K) | ||
| ΔH° : | n/a | ||
| ΔHobs : | -12±0 (kcal/mole) | ||
| Ionic Strength: | n/a | ||
| not known | |||
| Protons Released: | n/a | ||
| ΔCp : | -0.31±n/a (kcal/mole) | ||
| Stoich. Param.: | 1.1 | ||
| ΔS° : | -0.0100± (kcal/mole-K) | ||
| Comments: | n/a | ||
| Citation |  Hoffman, JM; Grunau, A; Smith, AM; Paine, MJ; Rooney, CS; Ladbury, JE; Fisher, TE; Gutierrez, A; Wai, JS; Thomas, CM; Bamberger, DL; Barnes, JL; Williams, TM; Jones, JH Global effects of the energetics of coenzyme binding: NADPH controls the protein interaction properties of human cytochrome P450 reductase. Biochemistry45:1421-34 (2006) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| Cytochrome P450 Reductase (CPR) | |||
| Source: | Human fibroblast CPR (lacking the N-terminal membrane-anchoring region) and the functional FAD-binding domain were expressed in Escherichia coli BL21 (DE3). | ||
| Purity: | n/a | ||
| Prep. Method: | The recombinant His-tagged proteins were purified to homogeneity by nickel-agarose chromatography. The notable exception is the omission of the 2,5-ADP affinity resin step to avoid the unusual biphasic binding isotherms during ITC experiment. | ||
| Name: | NADPH--cytochrome P450 reductase | ||
| Synonyms: | CYPOR | Cytochrome P450 Reductase (CPR) | NADPH--cytochrome P450 reductase | NCPR_HUMAN | P450R | POR | ||
| Type: | Enzyme | ||
| Mol. Mass.: | 76675.22 | ||
| Organism: | Homo sapiens (Human) | ||
| Description: | P16435 | ||
| Residue: | 677 | ||
| Sequence: |
| ||
| BDBM11939 | |||
| Source: | Sigma-Aldrich | ||
| Purity: | n/a | ||
| Prep. Method: | n/a | ||
| Name | BDBM11939 | ||
| Synonyms: | NADP+ | beta-nicotinamide adenine dinucleotide phosphate, oxidized form | ||
| Type | Nucleoside or nucleotide | ||
| Emp. Form. | C21H29N7O17P3 | ||
| Mol. Mass. | 744.4124 | ||
| SMILES | NC(=O)c1ccc[n+](c1)[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OC[C@H]2O[C@H]([C@H](OP(O)(O)=O)[C@@H]2O)n2cnc3c(N)ncnc23)[C@@H](O)[C@H]1O |r| | ||
| Structure | 
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