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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | HyHEL-5 Mutant(E50Q) | ||
| Syringe Reactant: | Lysozyme (bobwhite quail) | ||
| Meas. Tech.: | ITC and sedimentation equilibrium | ||
| Entry Date: | 12/13/00 | ||
| ΔG°: | -6.73±1.21 (kcal/mole) | ||
| pH: | 8±n/a | ||
| Log10Kb: | 5.08± n/a | ||
| Temperature: | 298.15±n/a (K) | ||
| ΔH° : | n/a | ||
| ΔHobs: | n/a | ||
| Ionic Strength: | n/a | ||
| not known | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | n/a | ||
| ΔS° : | n/a | ||
| Comments: | affinity was too low to measured through ITC | ||
| Citation |  Wibbenmeyer, JA; Schuck, P; Smith-Gill, SJ; Willson, RC Salt links dominate affinity of antibody HyHEL-5 for lysozyme through enthalpic contributions. J Biol Chem274:26838-42 (1999) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| HyHEL-5 Mutant(E50Q) | |||
| Source: | Site-directed mutagenesis of recombinant HyHEL-5 Fab | ||
| Purity: | 95% | ||
| Prep. Method: | Refolded protein was purified by Q Sepharose and nickel-charged chelating Sepharose Fast Flow Chromatography | ||
| Name: | HyHEL-5 Mutant(E50Q) | ||
| Synonyms: | HyHEL-5 Fab mutant (E50Q) | ||
| Type: | Antibody | ||
| Mol. Mass.: | 23011.87 | ||
| Organism: | Mus musculus (mouse) | ||
| Description: | mutant of murine monoclonal antibody HyHEL-5 to hen egg lysozyme | ||
| Residue: | 215 | ||
| Sequence: |
| ||
| Lysozyme (bobwhite quail) | |||
| Source: | Prepared from eggs purchased from Stevenson's Game Bird Farm (Riverside, TX) | ||
| Purity: | n/a | ||
| Prep. Method: | n/a | ||
| Name: | Lysozyme (bobwhite quail) | ||
| Synonyms: | LYSC_COLVI | LYZ | bobwhite quail lysozyme(BQL) | ||
| Type: | Enzyme | ||
| Mol. Mass.: | 14279.53 | ||
| Organism: | Colinus virginianus | ||
| Description: | hydrolase | ||
| Residue: | 129 | ||
| Sequence: |
| ||
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