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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | Glyoxylate Regulatory Protein (C-AllR) | ||
| Syringe Reactant: | BDBM19472 | ||
| Meas. Tech.: | Isothermal Titration Calorimetry | ||
| Entry Date: | 12/10/07 | ||
| ΔG°: | -4.43± (kJ/mole) | ||
| pH: | 7.4±n/a | ||
| Log10Kb: | 5.04± 4.48 | ||
| Temperature: | 298.15±n/a (K) | ||
| ΔH° : | n/a | ||
| ΔHobs : | -34.3± (kJ/mole) | ||
| Ionic Strength: | n/a | ||
| no | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | 0.5 | ||
| ΔS° : | -0.1011± (kJ/mole-K) | ||
| Comments: | n/a | ||
| Citation |  Lorca, GL; Ezersky, A; Lunin, VV; Walker, JR; Altamentova, S; Evdokimova, E; Vedadi, M; Bochkarev, A; Savchenko, A Glyoxylate and pyruvate are antagonistic effectors of the Escherichia coli IclR transcriptional regulator. J Biol Chem282:16476-91 (2007) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| Glyoxylate Regulatory Protein (C-AllR) | |||
| Source: | His-tagged fusion protein was overexpressed and purified in E. coli. | ||
| Purity: | n/a | ||
| Prep. Method: | The His6 tag was cleaved from the protein by tobacco etch virus protease. The cleaved protein was then resolved by flowing the mixture through a second Ni2+ column. The purified proteins were dialyzed and concentrated. | ||
| Name: | Glyoxylate Regulatory Protein (C-AllR) | ||
| Synonyms: | ALLR_ECOLI | Negative regulator of allantoin and glyoxylate utilization operons | Transcriptional regulator AllR, C-terminal domain | allR | glxA3 | ybbU | ||
| Type: | C-terminal effector-binding domain | ||
| Mol. Mass.: | 29267.82 | ||
| Organism: | Escherichia coli | ||
| Description: | P0ACN4 | ||
| Residue: | 271 | ||
| Sequence: |
| ||
| BDBM19472 | |||
| Source: | n/a | ||
| Purity: | n/a | ||
| Prep. Method: | n/a | ||
| Name | BDBM19472 | ||
| Synonyms: | 2-oxoacetic acid | Glyoxylate | Oxoethanoic acid | glyoxylic acid | oxaldehydic acid | ||
| Type | Small organic molecule | ||
| Emp. Form. | C2H2O3 | ||
| Mol. Mass. | 74.0355 | ||
| SMILES | OC(=O)C=O | ||
| Structure | 
| ||
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