Reaction Details | |||
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Cell Reactant: | BirA Bifunctional Protein | ||
Syringe Reactant: | BDBM19727 | ||
Meas. Tech.: | Isothermal Titration Calorimetry | ||
Entry Date: | 12/19/07 | ||
ΔG°: | -9.70±0.200 (kcal/mole) | ||
pH: | 7.5±0.2 | ||
Log10Kb: | 7.22± 6.40 | ||
Temperature: | 293.15±0.1 (K) | ||
ΔH° : | n/a | ||
ΔHobs : | -19.9±0.300 (kcal/mole) | ||
Ionic Strength: | n/a | ||
no | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | n/a | ||
ΔS° : | -0.0300±0 (kcal/mole-K) | ||
Comments: | n/a | ||
Citation | Brown, PH; Beckett, D Use of binding enthalpy to drive an allosteric transition. Biochemistry44:3112-21 (2005) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
BirA Bifunctional Protein | |||
Source: | The BirA variants were expressed in the Escherichia coli strain JM109 transformed with the plasmid pBtac2 carrying the E. coli birA gene (wild-type or mutant) under transcriptional control of the tac | ||
Purity: | >95% | ||
Prep. Method: | The separation and purification of BirA from other cellular proteins was carried out in NiNTA column and Sepharose chromatography steps. | ||
Name: | Bifunctional ligase/repressor BirA | ||
Synonyms: | BIRA_ECOLI | Biotin operon repressor | Biotin protein ligase (BPL) | Biotin--[acetyl-CoA-carboxylase] synthetase | BirA Bifunctional Protein | bioR | birA | dhbB | ||
Type: | Repressor protein | ||
Mol. Mass.: | 35313.58 | ||
Organism: | Escherichia coli | ||
Description: | n/a | ||
Residue: | 321 | ||
Sequence: |
| ||
BDBM19727 | |||
Source: | from RNA-Tec (Leuven, Belgium) | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name | BDBM19727 | ||
Synonyms: | (4S)-4-{5-[({[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}sulfonyl)amino]-5-oxopentyl}-hexahydro-1H-thieno[3,4-d]imidazolidin-2-one | 5-O-[N-(biotinoyl)sulfamoyl]adenosine | btn-SA | ||
Type | n/a | ||
Emp. Form. | C20H28N8O8S2 | ||
Mol. Mass. | 572.615 | ||
SMILES | Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COS(=O)(=O)NC(=O)CCCC[C@@H]2SCC3NC(=O)NC23)[C@@H](O)[C@H]1O |r| | ||
Structure |
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