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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | Rho Transcription Termination Factor | ||
| Syringe Reactant: | BDBM22600 | ||
| Meas. Tech.: | Isothermal Titration Calorimetry | ||
| Entry Date: | 06/14/08 | ||
| ΔG°: | -25.1±0.334 (kJ/mole) | ||
| pH: | 7.9±n/a | ||
| Log10Kb: | 4.39± 3.48 | ||
| Temperature: | 299.15±n/a (K) | ||
| ΔH° : | n/a | ||
| ΔHobs : | -3.30±3.01 (kJ/mole) | ||
| Ionic Strength: | n/a | ||
| no | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | 6 | ||
| ΔS° : | 0.0729±0.0112 (kJ/mole-K) | ||
| Comments: | The stoichiometry parameter was fixed to six, or for the two site model, two integers with a sum of six. | ||
| Citation |  Brogan, AP; Widger, WR; Bensadek, D; Riba-Garcia, I; Gaskell, SJ; Kohn, H Development of a technique to determine bicyclomycin-rho binding and stoichiometry by isothermal titration calorimetry and mass spectrometry. J Am Chem Soc127:2741-51 (2005) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| Rho Transcription Termination Factor | |||
| Source: | The open reading frame encoding the full-length Escherichia coli Rho protein was PCR amplified and cloned. The protein was overexpressed in E. coli strain BL21 (pLysS). | ||
| Purity: | 99% | ||
| Prep. Method: | The protein was twice purified over a POROS-HS column (Perseptive Biosystems) then further purified with two successive Sephacryl S-300 (Amersham) sizing columns. The protein at this stage was more than 99% pure as judged by SDS-PAGE. | ||
| Name: | Rho Transcription Termination Factor | ||
| Synonyms: | ATP-dependent helicase rho | RHO_ECOLI | nitA | psuA | rho | rnsC | sbaA | tsu | ||
| Type: | Enzyme | ||
| Mol. Mass.: | 47006.70 | ||
| Organism: | Escherichia coli (strain K12) | ||
| Description: | Rho is a hexameric RNA/DNA helicase/translocase. | ||
| Residue: | 419 | ||
| Sequence: |
| ||
| BDBM22600 | |||
| Source: | n/a | ||
| Purity: | n/a | ||
| Prep. Method: | n/a | ||
| Name | BDBM22600 | ||
| Synonyms: | (1S,6R)-6-hydroxy-5-methylidene-1-[(1S,2S)-1,2,3-trihydroxy-2-methylpropyl]-2-oxa-7,9-diazabicyclo[4.2.2]decane-8,10-dione | Bicyclomycin | CGP 354E | FR 1881 | bicozamycin | ||
| Type | Anti-Bacterial Agent | ||
| Emp. Form. | C12H18N2O7 | ||
| Mol. Mass. | 302.2805 | ||
| SMILES | C[C@](O)(CO)[C@H](O)[C@@]12NC(=O)[C@@](O)(NC1=O)C(=C)CCO2 |TLB:10:9:13.14:20.16.19.18,THB:15:14:8.9:20.16.19.18,17:16:13.14:8.9| | ||
| Structure | 
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