Reaction Details | |||
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Cell Reactant: | PLD beta C2 | ||
Syringe Reactant: | BDBM14 | ||
Meas. Tech.: | ITC and CD | ||
Entry Date: | 12/04/00 | ||
ΔG°: | -35.7± (kJ/mole) | ||
pH: | 7.5±n/a | ||
Log10Kb: | 6.10± n/a | ||
Temperature: | 303.15±n/a (K) | ||
ΔH° : | 0.588± (kJ/mole) | ||
ΔHobs : | 0.588± (kJ/mole) | ||
Ionic Strength: | n/a | ||
n/a | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | n/a | ||
ΔS° : | 0.1192± (kJ/mole-K) | ||
Comments: | PLD beta has two classes of binding sites | ||
Citation | Zheng, L; Krishnamoorthi, R; Zolkiewski, M; Wang, X Distinct Ca2+ binding properties of novel C2 domains of plant phospholipase dalpha and beta. J Biol Chem275:19700-6 (2000) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
PLD beta C2 | |||
Source: | poly-His-fused C2 proteins were expressed in E. coli BL21(DE3) | ||
Purity: | n/a | ||
Prep. Method: | C2 proteins without the tag were refolded by passing through a Sephacryl S-100 gel filtration column.Proteins were dissolved in Chelex-100 treated buffer | ||
Name: | PLD beta C2 | ||
Synonyms: | PLD beta C2 domain | PLDB1_ARATH | PLDBETA1 | ||
Type: | Protein | ||
Mol. Mass.: | 121118.74 | ||
Organism: | Arabidopsis thaliana | ||
Description: | P93733 | ||
Residue: | 1083 | ||
Sequence: |
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BDBM14 | |||
Source: | Fisher Scientific | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name | BDBM14 | ||
Synonyms: | Ca2+ | calcium | ||
Type | Metal Ion | ||
Emp. Form. | Ca | ||
Mol. Mass. | 40.077 | ||
SMILES | [Ca++] | ||
Structure |
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