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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | ferricytochrome c peroxidase (CcP) | ||
| Syringe Reactant: | iso-1-cytochrome c (Cc) | ||
| Meas. Tech.: | ITC | ||
| Entry Date: | 12/15/00 | ||
| ΔG°: | -31.1±1.26 (kJ/mole) | ||
| pH: | 6±n/a | ||
| Log10Kb: | n/a | ||
| Temperature: | 279.15±n/a (K) | ||
| ΔH° : | -12.2±0.420 (kJ/mole) | ||
| ΔHobs : | -5.88± (kJ/mole) | ||
| Ionic Strength: | n/a | ||
| yes | |||
| Protons Released: | 0.6 | ||
| ΔCp : | -0.9072±0.2604 (kJ/mole) | ||
| Stoich. Param.: | 1 | ||
| ΔS° : | 0.0903±0.00450 (kJ/mole-K) | ||
| Comments: | test the effect of temperature on binding | ||
| Citation |  Wang, X; Pielak, GJ Equilibrium thermodynamics of a physiologically-relevant heme-protein complex. Biochemistry38:16876-81 (1999) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
| ferricytochrome c peroxidase (CcP) | |||
| Source: | recombinant yeast CcP was expressed and purified from E.coli | ||
| Purity: | n/a | ||
| Prep. Method: | CcP and Cc were dialyzed (Slide -A_Lyzer membrane) against buffer in the same beaker for > 36 h with three buffer changes | ||
| Name: | ferricytochrome c peroxidase (CcP) | ||
| Synonyms: | n/a | ||
| Type: | Enzyme | ||
| Mol. Mass.: | 40387.06 | ||
| Organism: | Saccharomyces cerevisiae | ||
| Description: | A0A0D3U836 | ||
| Residue: | 362 | ||
| Sequence: |
| ||
| iso-1-cytochrome c (Cc) | |||
| Source: | purified from yeast | ||
| Purity: | n/a | ||
| Prep. Method: | CcP and Cc were dialyzed (Slide -A_Lyzer membrane) against buffer in the same beaker for > 36 h with three buffer changes | ||
| Name: | iso-1-cytochrome c (Cc) | ||
| Synonyms: | n/a | ||
| Type: | Protein | ||
| Mol. Mass.: | 12191.93 | ||
| Organism: | Saccharomyces cerevisiae | ||
| Description: | A0A0C6AZG8 | ||
| Residue: | 109 | ||
| Sequence: |
| ||
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