ITC Summary

Reaction Details

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Cell Reactant:

Grb2-SH2

Syringe Reactant:

pY317 (phosphotyrosine polypeptide)

Meas. Tech.:

ITC

Entry Date:

11/29/00

ΔG°:

-37.5±0.672 (kJ/mole)

pH:

7.5±n/a

Log₁₀Kb:

6.78± 6.25

Temperature:

288.15±n/a (K)

ΔH° :

-29.4±1.34 (kJ/mole)

ΔH_obs :

-29.4± (kJ/mole)

Ionic Strength:

n/a

Corrected for ΔH_ioniz:

yes

Protons Released:

ΔC_p :

-0.6132±n/a (kJ/mole)

Stoich. Param.:

n/a

ΔS° :

0.028±0.00700 (kJ/mole-K)

Comments:

n/a

Citation

McNemar, C; Snow, ME; Windsor, WT; Prongay, A; Mui, P; Zhang, R; Durkin, J; Le, HV; Weber, PC Thermodynamic and structural analysis of phosphotyrosine polypeptide binding to Grb2-SH2. Biochemistry36:10006-14 (1997) [PubMed] Article

More Info.:

Get all data from this article , ITC RUN data , Solution Info , Instrument Info

Grb2-SH2

Source:

DNA construct for the Grb2-SH2 domain was expressed in E.coli

Purity:

>98%

Prep. Method:

Mono-Q and Mono-S columns

Name:

Growth factor receptor-bound protein 2

Synonyms:

Type:

Protein

Mol. Mass.:

25205.04

Organism:

Homo sapiens (Human)

Description:

P62993

Residue:

217

Sequence:

MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPW
FFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFL
WVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRG
DFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV

pY317 (phosphotyrosine polypeptide)

Source:

AnaSpec Inc. (San Jose, CA)

Purity:

n/a

Prep. Method:

Fmoc solid-phase peptide synthesis

Name:

pY317 (phosphotyrosine polypeptide)

Synonyms:

n/a

Type:

Peptide

Mol. Mass.:

708.76

Organism:

n/a

Description:

phosphotyrosine polypeptide

Residue:

Sequence:

SYVNVQ

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