ITC Summary

Reaction Details

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Cell Reactant:

sc1E3

Syringe Reactant:

hIL-1beta

Meas. Tech.:

ITC

Entry Date:

12/05/00

ΔG°:

-46.2±0.420 (kJ/mole)

pH:

7±n/a

Log₁₀Kb:

7.67± 6.89

Temperature:

310.15±n/a (K)

ΔH° :

-93.2±1.68 (kJ/mole)

ΔH_obs :

-92.8± (kJ/mole)

Ionic Strength:

n/a

Corrected for ΔH_ioniz:

yes

Protons Released:

0.17

ΔC_p :

-1.19±0.349 (kJ/mole)

Stoich. Param.:

n/a

ΔS° :

-0.154±0.00500 (kJ/mole-K)

Comments:

n/a

Citation

Chrunyk, BA; Rosner, MH; Cong, Y; McColl, AS; Otterness, IG; Daumy, GO Inhibiting protein-protein interactions: a model for antagonist design Biochemistry39:7092-7099 (2000)

More Info.:

Get all data from this article , ITC RUN data , Solution Info , Instrument Info

sc1E3

Source:

Protein was expressed in E.coli consisted of the C-terminal end of the VL of the mAb1E3 joined to the N-terminal end of the VH by a linker.

Purity:

n/a

Prep. Method:

Cell suspension was purified by bound to anti-Flag M2 affinity column, and then a HiLoad Superdex 75 16/60 column

Name:

sc1E3

Synonyms:

n/a

Type:

Single Chain Antibody

Mol. Mass.:

358.43

Organism:

Escherichia coli

Description:

single chain antibody that blocked binding between IL-1beta and IL-1 receptor

Residue:

Sequence:

n/a

hIL-1beta

Source:

n/a

Purity:

n/a

Prep. Method:

n/a

Name:

Interleukin-1 beta

Synonyms:

Type:

Cytokine

Mol. Mass.:

30734.47

Organism:

Homo sapiens (Human)

Description:

P01584

Residue:

269

Sequence:

MAEVPELASEMMAYYSGNEDDLFFEADGPKQMKCSFQDLDLCPLDGGIQLRISDHHYSKG
FRQAASVVVAMDKLRKMLVPCPQTFQENDLSTFFPFIFEEEPIFFDTWDNEAYVHDAPVR
SLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKE
KNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYIST
SQAENMPVFLGGTKGGQDITDFTMQFVSS

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