| Reaction Details |
|---|
 | Report a problem with these data |
| Target | DNA topoisomerase 4 subunit A [V210L]/B |
|---|
| Ligand | BDBM21686 |
|---|
| Substrate/Competitor | Kinetoplast DNA |
|---|
| Meas. Tech. | DNA Topoisomerase IV Assay |
|---|
| pH | 7.5±n/a |
|---|
| Temperature | 303.15±n/a K |
|---|
| IC50 | >500000±n/a nM |
|---|
| Citation |  Butler, MM; Lamarr, WA; Foster, KA; Barnes, MH; Skow, DJ; Lyden, PT; Kustigian, LM; Zhi, C; Brown, NC; Wright, GE; Bowlin, TL Antibacterial activity and mechanism of action of a novel anilinouracil-fluoroquinolone hybrid compound. Antimicrob Agents Chemother51:119-27 (2007) [PubMed] Article |
|---|
| More Info.: | Get all data from this article, Solution Info, Assay Method |
|---|
| |
| DNA topoisomerase 4 subunit A [V210L]/B |
|---|
| Name: | DNA topoisomerase 4 subunit A [V210L]/B |
|---|
| Synonyms: | DNA Topoisomerase IV |
|---|
| Type: | Protein Complex |
|---|
| Mol. Mass.: | n/a |
|---|
| Description: | The parC and parE subunits of B. subtilis topoisomerase IV were purified from recombinant strains and combined to form active heterodimers. |
|---|
| Components: | This complex has 2 components. |
|---|
| Component 1 |
| Name: | DNA topoisomerase 4 subunit A [V210L] |
|---|
| Synonyms: | DNA Topoisomerase IV Chain A | PARC_BACSU | grlA | parC |
|---|
| Type: | Enzyme Subunit |
|---|
| Mol. Mass.: | 91336.01 |
|---|
| Organism: | Bacillus subtilis |
|---|
| Description: | Q45066[V210L] |
|---|
| Residue: | 806 |
|---|
| Sequence: | MSQPELFHDLPLEEVIGDRFGRYSKYIIQDRALPDARDGLKPVQRRILYAMHTDGNTFDK
NFRKAAKTVGNVIGNYHPHGDSSVYEAMVRMSQDWKVRNVLIEMHGNNGSIDGDPPAAMR
YTEARLSPIASELLRDIDKNTVEFVPNFDDTSKEPVVLPAMFPNLLVNGSTGISAGYATD
IPPHHLGEVIDAVIKRIQMPSCSVDELMELIKGPDFPTGGIIQGVDGIRKAYETGKGKII
IRGKAEIETIRGGREQIVITEIPFEVNKANLVKKMDEFRIDKKVEGISEVRDETDRTGLR
VVIELKKEADAKGILNFLYKNTDLQITYNFNMVAIHNRRPMLMSLPSILDAYIGHQKEVV
TNRSVYELQKAKDRHHIVEGLMKALSILDEVIATIRSSSDKRDAKNNLIAKYEFTEPQAE
AIVSLQLYRLTNTDITALKEEAEELGKKIEELESILSNDKKLLKVITNSLKALKKKYADT
RRSVIEEKIEEIKINLEVMVASEDVYVTVTKDGYLKRTSQRSFAASNGQDFGMKDTDRML
HQFEMNTTDVLLLFTNKGSYIYCPVHQLPDIRWKDMGQHFSNLITIDRDETIVKAIPIKE
FDPSAYLLFFTKNGMVKKTELTHYKAQRYSKALVALNLKGEDELIDVHVTNGESQIFMAT
HLGYGLWFGEDEVNVVGARAAGVKGINLKEDDFVVSGEILQQSDSIVLFTQRGAVKRMSL
SEFEKTSRAKRGVVMLRELKKNPHRVVALFACGLEQRLMAETEKGDRKELQTKELRTNDR
YSNGSFFFDEEESGKVTAVWRLHTEQ
|
|
|
|---|
| Component 2 |
| Name: | DNA topoisomerase 4 subunit B |
|---|
| Synonyms: | DNA Topoisomerase IV Chain B | PARE_BACSU | grlB | parE |
|---|
| Type: | Enzyme Subunit |
|---|
| Mol. Mass.: | 73006.28 |
|---|
| Organism: | Bacillus subtilis |
|---|
| Description: | n/a |
|---|
| Residue: | 655 |
|---|
| Sequence: | MARKQQFDYNEDAIQVLEGLEAVRKRPGMYIGSTDARGLHHLVYEIVDNSVDEVLAGHGD
HIIVKIHKDNSISVQDRGRGMPTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGV
GASVVNALSEWLTVTIERDGFVYQQRFENGGKPVTSLEKIGKTKKTGTLTHFKPDPTMFS
TTTYNFETLSERLRESAFLLKGLKIELIDERNDQREVFYYENGIEAFVAYLNEEKDVLSE
VVSFEGEHHSIEVDFAFQFNDGYSENILSFVNNVRTKDGGTHESGAKTAMTRAFNEYARK
VALLKEKDKNLEGTDIREGLSAIISVRIPEELLQFEGQTKGKLGTSEARSAVDAIVSEQL
AYFLEENRDTATLLVKKAIKASQAREAARKAREEARSGKKRKKSEATLSGKLTPAGSRNP
AKNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIHAIG
GGVGADFSIDDINYDKIIIMTDADTDGAHIQVLLLTFFYRYMKPLIEHGKVFIALPPLYK
VSKGSGKKEIIEYAWSDEEMGDVLKKVGKGYTIQRYKGLGEMNADQLWETTMNPESRTLV
RVKIDDAARVERRVTTLMGDKVEPRRKWIEKNVAFGLDEESNILENENLSVAEEV
|
|
|
|---|
| BDBM21686 |
|---|
| Kinetoplast DNA |
|---|
| Name: | Kinetoplast DNA |
|---|
| Synonyms: | KDNA (catenated) |
|---|
| Type: | Duplex DNA |
|---|
| Mol. Mass.: | 358.43 |
|---|
| Organism: | Crithidia fasciculata |
|---|
| Description: | n/a |
|---|
| Residue: | 3 |
|---|
| Sequence: | |
|---|
Search and Browse
