Ki Summary

Reaction Details

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Target

Serine/threonine-protein kinase Chk1

Ligand

BDBM33213

Substrate/Competitor

biotinylated CDC25C peptide

Meas. Tech.

CHK Kinase Inhibition Assay

7.5±n/a

Temperature

296.15±n/a K

IC50

390±n/a nM

Citation

Matthews, TP; Klair, S; Burns, S; Boxall, K; Cherry, M; Fisher, M; Westwood, IM; Walton, MI; McHardy, T; Cheung, KM; Van Montfort, R; Williams, D; Aherne, GW; Garrett, MD; Reader, J; Collins, I Identification of inhibitors of checkpoint kinase 1 through template screening. J Med Chem52:4810-9 (2009) [PubMed] Article

More Info.:

Get all data from this article, Inhibition_Run data, Solution Info, Assay Method

Serine/threonine-protein kinase Chk1

Name:

Serine/threonine-protein kinase Chk1

Synonyms:

CHEK1 | CHK1 | CHK1 checkpoint homolog | CHK1_HUMAN | Checkpoint kinase-1 (CHK1)

Type:

Enzyme Catalytic Domain

Mol. Mass.:

54443.02

Organism:

Homo sapiens (Human)

Description:

gi_166295192

Residue:

476

Sequence:

MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINK
MLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVY
LHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLK
RREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLA
LLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDF
SPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLL
GTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRR
NNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

BDBM33213

biotinylated CDC25C peptide

Name:

biotinylated CDC25C peptide

Synonyms:

n/a

Type:

biotinylated peptide

Mol. Mass.:

3148.69

Organism:

n/a

Description:

n/a

Residue:

Sequence:

Biotin-KKKVSRSGLYRSPSMPENLNRPR