| Reaction Details |
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 | Report a problem with these data |
| Target | Cyclin-dependent kinase/G2/mitotic-specific cyclin- 1 |
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| Ligand | BDBM33212 |
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| Substrate/Competitor | Rb (Ser780) Biotinylated Peptide |
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| Meas. Tech. | CDK1 Inhibition Assay |
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| pH | 7.5±n/a |
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| Temperature | 296.15±n/a K |
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| Comments | 3% inhibition @ 100 uM. |
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| Citation |  Matthews, TP; Klair, S; Burns, S; Boxall, K; Cherry, M; Fisher, M; Westwood, IM; Walton, MI; McHardy, T; Cheung, KM; Van Montfort, R; Williams, D; Aherne, GW; Garrett, MD; Reader, J; Collins, I Identification of inhibitors of checkpoint kinase 1 through template screening. J Med Chem52:4810-9 (2009) [PubMed] Article |
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| More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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| Cyclin-dependent kinase/G2/mitotic-specific cyclin- 1 |
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| Name: | Cyclin-dependent kinase/G2/mitotic-specific cyclin- 1 |
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| Synonyms: | CDK1/B | CDK1/Cyclin B | CDK1/CyclinB | Cyclin B/Dependent Kinase 1 (CDK1) | Cyclin-Dependent Kinase 1 (CDK1) | Cyclin-dependent kinase 1/cyclin B1 | Cyclin-dependent kinase/G2/mitotic-specific cyclin 1 | cdk1 |
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| Type: | Protein Complex |
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| Mol. Mass.: | n/a |
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| Description: | CDK1/Cyclin B complexes were purified from insect cells co-infected with baculovirus vectors containing each of the components. |
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| Components: | This complex has 2 components. |
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| Component 1 |
| Name: | Cyclin-dependent kinase 1 |
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| Synonyms: | CDC2 | CDC28A | CDK1 | CDK1_HUMAN | CDKN1 | Cell division control protein 2 homolog | Cell division protein kinase 1 | Cyclin-dependent kinase 1 (CDK1) | P34CDC2 | p34 protein kinase |
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| Type: | Enzyme Subunit |
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| Mol. Mass.: | 34101.08 |
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| Organism: | Homo sapiens (Human) |
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| Description: | P06493 |
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| Residue: | 297 |
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| Sequence: | MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRH
PNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCH
SRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSAR
YSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNT
FPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM
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| Component 2 |
| Name: | G2/mitotic-specific cyclin-B1 |
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| Synonyms: | CCNB | CCNB1 | CCNB1_HUMAN |
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| Type: | Enzyme Subunit |
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| Mol. Mass.: | 48340.95 |
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| Organism: | Homo sapiens (Human) |
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| Description: | P14635 |
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| Residue: | 433 |
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| Sequence: | MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKM
PMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPI
LVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAYLRQ
LEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVP
KKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRP
LPLHFLRRASKIGEVDVEQHTLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGE
WTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNS
ALVQDLAKAVAKV
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| BDBM33212 |
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| Rb (Ser780) Biotinylated Peptide |
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| Name: | Rb (Ser780) Biotinylated Peptide |
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| Synonyms: | n/a |
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| Type: | Biotinylated peptide |
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| Mol. Mass.: | 723.86 |
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| Organism: | n/a |
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| Description: | Peptide contains residues surrounding serine 780 of Rb. Only the peptide core sequence is shown here. |
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| Residue: | 7 |
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| Sequence: | |
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