Reaction Details |
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Target | Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B |
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Ligand | BDBM7369 |
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Substrate/Competitor | Histone H1 |
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Meas. Tech. | Kinase Inhibition Assay |
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Temperature | 303.15±n/a K |
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IC50 | 400±n/a nM |
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Comments | extracted |
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Citation | Mettey, Y; Gompel, M; Thomas, V; Garnier, M; Leost, M; Ceballos-Picot, I; Noble, M; Endicott, J; Vierfond, JM; Meijer, L Aloisines, a new family of CDK/GSK-3 inhibitors. SAR study, crystal structure in complex with CDK2, enzyme selectivity, and cellular effects. J Med Chem46:222-36 (2003) [PubMed] Article |
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More Info.: | Get all data from this article, Solution Info, Assay Method |
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Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B |
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Name: | Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B |
Synonyms: | CDK1/Cyclin B | Cyclin-Dependent Kinase 1 (CDK1) | p34cdc2/Cyclin B |
Type: | Protein Complex |
Mol. Mass.: | n/a |
Description: | p34cdc2/cyclin B was purified from M-phase starfish oocytes by affinity chromatography. |
Components: | This complex has 2 components. |
Component 1 |
Name: | Cyclin-dependent kinase 1 |
Synonyms: | CDK1_ORYLA | Cell division control protein 2 (cdc2) | P34 protein kinase | cdc2 | cdk1 |
Type: | Enzyme Subunit |
Mol. Mass.: | 34693.35 |
Organism: | Marthasterias glacialis (starfish) |
Description: | Q9DGD3 {empty hard to find) |
Residue: | 303 |
Sequence: | MEDYVKIEKIGEGTYGVVYKGRHKSTGQVVAMKKIRLESEEEGVPSTAVREVSLLQELKH
PNVVRLLDVLMQESRLYLIFEFLSMDLKKYLDSIPSGQYMDPMLVKSYLYQILEGIYFCH
RRRVLHRDLKPQNLLIDNKGVIKLADFGLARAFGVPVRVYTHEVVTLWYRAPEVLLGSPR
YSTPVDVWSTGTIFAELATKKPLFHGDSEIDQLFRIFRTLGTPNNDVWPDVESLPDYKNT
FPKWKEGSLSSMVKNLDKNGLDLLAKMLIYNPPKRISAREAMTHPYFDDLDKSTLPAACI
NGV
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Component 2 |
Name: | G2/mitotic-specific cyclin-B |
Synonyms: | CCNB_MARGL | Cyclin B | G2/mitotic-specific cyclin-B1 |
Type: | Enzyme Subunit |
Mol. Mass.: | 43790.28 |
Organism: | Marthasterias glacialis (starfish) |
Description: | n/a |
Residue: | 388 |
Sequence: | MLNGENVDSRIMGKVATRASSKGVKSTLGTRGALENISNVARNNLQAGAKKELVKAKRGM
TKSKATSSLQSVMGLNVEPMEKAKPQSPEPMDMSEINSALEAFSQNLLEGVEDIDKNDFD
NPQLCSEFVNDIYQYMRKLEREFKVRTDYMTIQEITERMRSILIDWLVQVHLRFHLLQET
LFLTIQILDRYLEVQPVSKNKLQLVGVTSMLIAAKYEEMYPPEIGDFVYITDNAYTKAQI
RSMECNILRRLDFSLGKPLCIHFLRRNSKAGGVDGQKHTMAKYLMELTLPEYAFVPYDPS
EIAAAALCLSSKILEPDMEWGTTLVHYSAYSEDHLMPIVQKMALVLKNAPTAKFQAVRKK
YSSAKFMNVSTISALTSSTVMDLADQMC
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BDBM7369 |
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Histone H1 |
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Name: | Histone H1 |
Synonyms: | n/a |
Type: | Other Protein Type |
Mol. Mass.: | 358.43 |
Organism: | Bos taurus (bovine) |
Description: | n/a |
Residue: | 3 |
Sequence: | |