| Reaction Details |
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 | Report a problem with these data |
| Target | cGMP-specific 3',5'-cyclic phosphodiesterase [1-662,679-875] |
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| Ligand | BDBM14390 |
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| Substrate/Competitor | BDBM14391 |
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| Meas. Tech. | Enzymatic Assay |
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| pH | 7.8±n/a |
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| Temperature | 297.15±n/a K |
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| IC50 | 5.9±1.5 nM |
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| Km | 54000±6000 nM |
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| kcat | 3.8±0.5 1/sec |
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| Citation |  Wang, H; Liu, Y; Huai, Q; Cai, J; Zoraghi, R; Francis, SH; Corbin, JD; Robinson, H; Xin, Z; Lin, G; Ke, H Multiple conformations of phosphodiesterase-5: implications for enzyme function and drug development. J Biol Chem281:21469-79 (2006) [PubMed] Article |
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| More Info.: | Get all data from this article, Solution Info, Assay Method |
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| cGMP-specific 3',5'-cyclic phosphodiesterase [1-662,679-875] |
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| Name: | cGMP-specific 3',5'-cyclic phosphodiesterase [1-662,679-875] |
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| Synonyms: | PDE5 | PDE5A | PDE5A_HUMAN | Phosphodiesterase Type 5 (PDE5A) H-loop deletion mutant | Phosphodiesterase Type 5 (PDE5A) Mutant (del 663-678) |
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| Type: | Enzyme Catalytic Domain |
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| Mol. Mass.: | 98047.67 |
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| Organism: | Homo sapiens (Human) |
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| Description: | Mutant was generated from wild-type clone and expressed in E. coli. |
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| Residue: | 859 |
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| Sequence: | MERAGPSFGQQRQQQQPQQQKQQQRDQDSVEAWLDDHWDFTFSYFVRKATREMVNAWFAE
RVHTIPVCKEGIRGHTESCSCPLQQSPRADNSAPGTPTRKISASEFDRPLRPIVVKDSEG
TVSFLSDSEKKEQMPLTPPRFDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLI
SADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEVSNNCIRLEWNKGIVGHVAALGEPL
NIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDE
KDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIIS
FMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTREHDANKINYMYAQYVKNTME
PLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGK
VKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETREL
QSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWIL
SVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGV
NNSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFF
ELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKE
LNIEPTDLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQAL
AEQQEKMLINGESGQAKRN
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| BDBM14390 |
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| BDBM14391 |
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| Name | BDBM14390 |
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| Synonyms: | 5-[2-ethoxy-5-(4-methyl-1-piperazinylsulfonyl)phenyl]-1-methyl-3-n-propyl-1,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one | 5-{2-ethoxy-5-[(4-methylpiperazine-1-)sulfonyl]phenyl}-1-methyl-3-propyl-1H,6H,7H-pyrazolo[4,3-d]pyrimidin-7-one | CHEMBL192 | SILDENAFIL CITRATE | Sildenafil | Sildenafil# | US11155558, Compound sildenafil | US11242347, Compound sildenafil | US11897890, Compound sildenafil | Viagra |
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| Type | Small organic molecule |
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| Emp. Form. | C22H30N6O4S |
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| Mol. Mass. | 474.576 |
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| SMILES | CCCc1nn(C)c2c1nc([nH]c2=O)-c1cc(ccc1OCC)S(=O)(=O)N1CCN(C)CC1 |
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| Structure | 
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