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TargetDihydrofolate Reductase (DHFR)
LigandBDBM18050
Substrate/CompetitorBDBM18044
Meas. Tech.Dihydrofolate Reductase (DHFR) Assay
pH7.4±n/a
Temperature303.15±n/a K
IC50 6.6±n/a nM
Citation Gangjee AZeng YTalreja TMcGuire JJKisliuk RLQueener SF Design and Synthesis of Classical and Nonclassical 6-Arylthio-2,4-diamino-5-ethylpyrrolo[2,3-d]pyrimidines as Antifolates. J Med Chem 50:3046-3053 (2007) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Dihydrofolate Reductase (DHFR)
Name:Bacterial dihydrofolate reductase
Synonyms:Bacterial dihydrofolate reductase | Tetrahydrofolate dehydrogenase
Type:Enzyme
Mol. Mass.:17991.61
Organism:Escherichia coli
Description:E. coli DHFR was expressed in BL21, and purified to homogeneity.
Residue:159
Sequence:
MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRPLPGRKNI
ILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVE
GDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR
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BDBM18050
BDBM18044
NameBDBM18050
Synonyms:2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)amino}phenyl)formamido]pentanedioic acid | CHEMBL34259 | METHOTREXATE | MTX | Methotrexate | cid_126941
TypeSmall organic molecule
Emp. Form.C20H22N8O5
Mol. Mass.454.4393
SMILESCN(Cc1cnc2nc(N)nc(N)c2n1)c1ccc(cc1)C(=O)N[C@@H](CCC(O)=O)C(O)=O |r|
Structure
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