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TargetDNA-(apurinic or apyrimidinic site) endonuclease
LigandBDBM26613
Substrate/CompetitorDNA Substrate
Meas. Tech.High-Throughput Screening and IC50 Determinations
pH7.5±n/a
Temperature295.15±n/a K
IC50 200±100 nM
Comments98% Inhibition uM.
Citation Seiple, LA; Cardellina, JH; Akee, R; Stivers, JT Potent inhibition of human apurinic/apyrimidinic endonuclease 1 by arylstibonic acids. Mol Pharmacol73:669-77 (2008) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
DNA-(apurinic or apyrimidinic site) endonuclease
Name:DNA-(apurinic or apyrimidinic site) endonuclease
Synonyms:APE | APE1 | APEX | APEX1 | APEX1_HUMAN | APX | Apurinic-apyrimidinic endonuclease 1 (APE-1) | Apurinic/apyrimidinic endonuclease 1 (APE1) | DNA-(apurinic or apyrimidinic site) lyase | HAP1 | REF1
Type:Protein
Mol. Mass.:35560.12
Organism:Homo sapiens (Human)
Description:P27695
Residue:318
Sequence:
MPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPALYEDPPDQKTSPSGKPA
TLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLSHQYWS
APSDKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLV
RLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGF
GELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKI
RSKALGSDHCPITLYLAL
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM26613
DNA Substrate
Name:DNA Substrate
Synonyms:Annealed double-stranded oligonucleotide with an abasic site.
Type:Fluorescent substrate
Mol. Mass.:1563.78
Organism:n/a
Description:The top strand is 5-FAM-GAG AA(F) ATA GTC GCG-3 containing the tetrahydrofuran synthetic abasic residue (F).
Residue:18
Sequence:
5-FAM-GAG AA(F) ATA GTC GCG-3