Ki Summary BindingDB logo
myBDB logout
Reaction Details
Report a problem with these data
TargetRibonuclease HI
LigandBDBM33963
Substrate/CompetitorRNA/DNA hybrid
Meas. Tech.FRET-Based RNase H Assay
pH8±n/a
Temperature293.15±n/a K
IC50 73000±n/a nM
Citation Wendeler, M; Lee, HF; Bermingham, A; Miller, JT; Chertov, O; Bona, MK; Baichoo, NS; Ehteshami, M; Beutler, J; O'Keefe, BR; Götte, M; Kvaratskhelia, M; Le Grice, S Vinylogous ureas as a novel class of inhibitors of reverse transcriptase-associated ribonuclease H activity. ACS Chem Biol3:635-44 (2008) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Ribonuclease HI
Name:Ribonuclease HI
Synonyms:RNH_ECOLI | RNase H | Ribonuclease H | Ribonuclease HI | dasF | herA | rnh | rnhA | sdrA
Type:Enzyme
Mol. Mass.:17601.33
Organism:Escherichia coli (strain K12)
Description:n/a
Residue:155
Sequence:
MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTNNRMELMAAIVALEALK
EHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVDLWQRLDAALGQHQIKWEW
VKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM33963
RNA/DNA hybrid
Name:RNA/DNA hybrid
Synonyms:n/a
Type:fluorescein-labeled RNA annealed to DNA
Mol. Mass.:2134.40
Organism:n/a
Description:It is a 18-nucleotide 3-fluorescein-labeled RNA annealed to a complementary 18-nucleotide 5-Dabcyl-modified DNA.
Residue:23
Sequence:
5-GAU CUG AGC CUG GGA GCU-fluorescein-3