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TargetDNA-directed RNA polymerase subunit alpha/beta/beta'/omega
LigandBDBM50000054
Substrate/Competitorn/a
Meas. Tech.ChEMBL_1334097 (CHEMBL3232507)
IC50 2800±n/a nM
Citation Sinha, BK; Cox, MG; Chignell, CF; Cysyk, RL Synthesis and biological properties of N2-substituted spin-labeled analogues of actinomycin D. J Med Chem22:1051-5 (1979) [PubMed]
More Info.:Get all data from this article,  Assay Method
 
DNA-directed RNA polymerase subunit alpha/beta/beta'/omega
Name:DNA-directed RNA polymerase subunit alpha/beta/beta'/omega
Synonyms:Bacterial DNA-directed RNA polymerase
Type:n/a
Mol. Mass.:n/a
Description:ASSAY_ID of ChEMBL is 1334097
Components:This complex has 4 components.
Component 1
Name:DNA-directed RNA polymerase subunit alpha
Synonyms:JW3257 | RNA polymerase subunit alpha | RNAP subunit alpha | RPOA_ECOLI | Transcriptase subunit alpha | pez | phs | rpoA | sez
Type:PROTEIN
Mol. Mass.:36497.18
Organism:Escherichia coli K-12
Description:ChEMBL_106172
Residue:329
Sequence:
MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
IDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGD
VEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIHSEEDERPIGRLLVDACYSPV
ERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVDLRDVRQP
EVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSL
TEIKDVLASRGLSLGMRLENWPPASIADE
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Component 2
Name:DNA-directed RNA polymerase subunit beta
Synonyms:Bacterial DNA-directed RNA polymerase | DNA-directed RNA polymerase beta chain | DNA-directed RNA polymerase subunit beta | RNA polymerase subunit beta | RNA polymerase subunit beta (β-RNAP) | RNAP subunit beta | RPOB_ECOLI | Transcriptase subunit beta | groN | nitB | rif | ron | rpoB | stl | stv | tabD
Type:Enzyme
Mol. Mass.:150595.80
Organism:Escherichia coli
Description:P0A8V2
Residue:1342
Sequence:
MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQ
SYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQ
EVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYR
GSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQME
LVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKD
YIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRL
SALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGIL
SKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLS
LGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTR
ERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVT
DEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQV
VSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKR
GGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERG
DVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVS
RDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEK
LLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDL
SEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDE
LKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKIN
PIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAK
LREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLK
LGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLG
GKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
ESFNVLLKEIRSLGINIELEDE
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Component 3
Name:DNA-directed RNA polymerase subunit beta'
Synonyms:JW3951 | RPOC_ECOLI | rpoC | tabB
Type:PROTEIN
Mol. Mass.:155165.00
Organism:Escherichia coli K-12
Description:ChEMBL_106172
Residue:1407
Sequence:
MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCAR
IFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIELASPTAHIWFLKSL
PSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQILTEEQYLDALEEFGDEFDAKM
GAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMILT
VLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQ
EAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPY
LRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKKMVEREEAVVWDILDEVIREHP
VLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEA
RALMMSTNNILSPANGEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAERLYRSG
LASLHARVKVRITEYEKDANGELVAKTSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKA
ISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAE
VAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFN
SIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGAR
KGLADTALKTANSGYLTRRLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVL
GRVTAEDVLKPGTADILVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYG
RDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKL
SNVKSVVNSSGKLVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANW
DPHTMPVITEVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKI
VDAQGNDVLIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPR
VADLFEARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFE
GERVERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQM
LRKATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEAPA
APQVTAEDASASLAELLNAGLGGSDNE
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Component 4
Name:DNA-directed RNA polymerase subunit omega
Synonyms:JW3624 | RNA polymerase omega subunit | RNAP omega subunit | RPOZ_ECOLI | Transcriptase subunit omega | rpoZ
Type:PROTEIN
Mol. Mass.:10232.42
Organism:Escherichia coli K-12
Description:ChEMBL_106172
Residue:91
Sequence:
MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKTTVIALREIEEGLIN
NQILDVRERQEQQEQEAAELQAVTAIAEGRR
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BDBM50000054
n/a
NameBDBM50000054
Synonyms:CHEMBL3228735
TypeSmall organic molecule
Emp. Form.C71H102N13O17
Mol. Mass.1409.6465
SMILES[H][C@@]12CCCN1C(=O)[C@H](NC(=O)[C@@H](NC(=O)c1ccc(C)c3oc4c(C)c(=O)c(NC5CC(C)(C)[N+]([O-])C(C)(C)C5)c(C(=O)N[C@H]5[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@]6([H])CCCN6C(=O)[C@H](NC5=O)C(C)C)c4nc13)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C2=O)C(C)C |r,wU:12.13,8.107,51.53,46.48,1.0,wD:45.46,72.79,87.94,82.89,64.66,^1:35,(36.21,-4.82,;37.96,-4.15,;36.92,-3.01,;37.68,-1.67,;39.19,-1.98,;39.37,-3.5,;40.71,-4.27,;40.71,-5.81,;42.04,-3.5,;43.37,-4.27,;45.91,-10.38,;47.24,-11.15,;44.57,-11.13,;44.56,-12.67,;45.89,-13.46,;47.22,-12.69,;45.88,-15,;44.55,-15.75,;44.53,-17.29,;45.86,-18.08,;45.84,-19.62,;47.2,-17.31,;48.52,-18.08,;49.85,-17.33,;51.16,-18.1,;51.14,-19.65,;52.48,-17.34,;53.82,-18.12,;52.49,-15.82,;53.82,-15.05,;55.16,-15.82,;55.15,-17.35,;56.47,-18.11,;57.24,-19.44,;55.7,-19.44,;57.81,-17.36,;59.14,-18.13,;57.81,-15.81,;58.9,-14.72,;59.31,-16.21,;56.48,-15.04,;51.16,-15.05,;51.18,-13.51,;49.86,-12.74,;52.52,-12.74,;52.52,-11.2,;53.86,-10.43,;53.87,-8.91,;55.19,-11.22,;56.53,-10.45,;56.53,-8.91,;57.86,-11.22,;57.85,-12.76,;59.19,-13.53,;56.52,-13.53,;59.2,-10.45,;60.53,-11.24,;59.21,-8.91,;57.87,-8.14,;60.54,-8.16,;60.54,-6.6,;61.9,-5.85,;59.21,-5.83,;57.88,-6.6,;59.23,-4.29,;60.88,-5.16,;60.49,-3.4,;60.01,-1.93,;58.47,-1.93,;57.99,-3.38,;56.65,-4.15,;56.65,-5.69,;55.32,-3.38,;53.98,-4.13,;51.19,-10.43,;49.86,-11.19,;55.32,-1.82,;53.99,-1.05,;56.66,-1.05,;49.86,-15.8,;48.53,-15.03,;47.21,-15.77,;43.24,-10.36,;43.25,-8.82,;41.9,-11.12,;40.57,-10.34,;40.59,-8.8,;39.24,-11.1,;39.22,-12.64,;40.55,-13.42,;37.89,-13.39,;37.9,-10.31,;36.57,-11.08,;37.91,-8.77,;39.26,-8.02,;36.58,-8,;36.6,-6.46,;35.27,-5.67,;37.95,-5.69,;39.26,-6.46,;42.02,-1.96,;43.36,-1.19,;40.69,-1.19,)|
Structure
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