| Reaction Details |
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 | Report a problem with these data |
| Target | Nitric oxide synthase, brain [D597N] |
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| Ligand | BDBM190667 |
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| Substrate/Competitor | n/a |
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| Meas. Tech. | Hemoglobin Capture Assay |
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| pH | 7.4±n/a |
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| Temperature | 310.15±n/a K |
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| Ki | 46.7±0.0 nM |
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| Comments | extracted |
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| Citation |  Li, H; Wang, HY; Kang, S; Silverman, RB; Poulos, TL Electrostatic Control of Isoform Selective Inhibitor Binding in Nitric Oxide Synthase. Biochemistry55:3702-7 (2016) [PubMed] Article |
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| More Info.: | Get all data from this article, Assay Method |
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| Nitric oxide synthase, brain [D597N] |
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| Name: | Nitric oxide synthase, brain [D597N] |
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| Synonyms: | Bnos | N-NOS | NC-NOS | NOS1_RAT | Neuronal nitric oxide synthase | Neuronal nitric oxide synthase mutant (D597N) | Nitric Oxide Synthase, brain Mutant (D597N) | Nos1 |
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| Type: | Enzyme |
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| Mol. Mass.: | 160571.01 |
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| Organism: | Rattus norvegicus (rat) |
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| Description: | Recombinant enzyme overexpressed in E. coli. |
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| Residue: | 1429 |
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| Sequence: | MEENTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQA
GDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTI
RVTQPLGPPTKAVDLSHQPSASKDQSLAVDRVTGLGNGPQHAQGHGQGAGSVSQANGVAI
DPTMKSTKANLQDIGEHDELLKEIEPVLSILNSGSKATNRGGPAKAEMKDTGIQVDRDLD
GKSHKAPPLGGDNDRVFNDLWGKDNVPVILNNPYSEKEQSPTSGKQSPTKNGSPSRCPRF
LKVKNWETDVVLTDTLHLKSTLETGCTEHICMGSIMLPSQHTRKPEDVRTKDQLFPLAKE
FLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQ
WSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLI
RYAGYKQPDGSTLGDPANVQFTEICIQQGWKAPRGRFDVLPLLLQANGNDPELFQIPPEL
VLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRNYCD
NSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIK
HMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTN
GTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHA
FDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKS
YKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVD
TLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKPNNSLISN
DRSWKRNKFRLTYVAEAPDLTQGLSNVHKKRVSAARLLSRQNLQSPKFSRSTIFVRLHTN
GNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWK
DESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKW
GKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTR
DGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFW
QQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPK
KYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVF
ISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDADEVFSS
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| BDBM190667 |
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| n/a |
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| Name | BDBM190667 |
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| Synonyms: | US10759791, Compound 14j | nNOS inhibitor, 5 |
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| Type | Small organic molecule |
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| Emp. Form. | C17H24N4 |
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| Mol. Mass. | 284.3993 |
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| SMILES | CNCCCc1cncc(CCc2cc(C)cc(N)n2)c1 |
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| Structure | 
|
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