| Reaction Details |
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 | Report a problem with these data |
| Target | Na(+)/H(+) exchange regulatory cofactor NHE-RF1 [133-300] |
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| Ligand | BDBM228845 |
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| Substrate/Competitor | n/a |
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| Meas. Tech. | Fluorescent Polarization Competition Assay |
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| pH | 7.4±0 |
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| Temperature | 296.15±0 K |
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| Kd | 2.4e+3± 4e+2 nM |
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| Citation |  Mamonova, T; Zhang, Q; Chandra, M; Collins, BM; Sarfo, E; Bu, Z; Xiao, K; Bisello, A; Friedman, PA Origins of PDZ Binding Specificity. A Computational and Experimental Study Using NHERF1 and the Parathyroid Hormone Receptor. Biochemistry56:2584-2593 (2017) [PubMed] Article |
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| More Info.: | Get all data from this article, Solution Info, Assay Method |
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| Na(+)/H(+) exchange regulatory cofactor NHE-RF1 [133-300] |
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| Name: | Na(+)/H(+) exchange regulatory cofactor NHE-RF1 [133-300] |
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| Synonyms: | NHERF | NHERF1 | NHRF1_HUMAN | Na+/H+ exchanger regulatory factor-1 PDZ domain (PDZ2) | SLC9A3R1 |
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| Type: | Protein |
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| Mol. Mass.: | 18472.23 |
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| Organism: | Homo sapiens (Human) |
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| Description: | PDZ2 domain of human NHERF1 (133-300 aa) |
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| Residue: | 168 |
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| Sequence: | NENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASG
LRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLN
GPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQ
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| BDBM228845 |
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| n/a |
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| Name | BDBM228845 |
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| Synonyms: | FITC-LQEEWETVM | FITC-PTHRct9 |
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| Type | Small organic molecule |
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| Emp. Form. | C72H88N12O23S2 |
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| Mol. Mass. | 1553.666 |
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| SMILES | CSCC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=S)Nc1ccc2c(c1)C(=O)OC21c2ccc(O)cc2Oc2cc(O)ccc12)[C@@H](C)O)C(C)C)C(O)=O |r| |
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| Structure | 
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