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Assay Method Information

Assay Name:  CDK Inhibition Assay
Description:  In vitro kinase assay using purified enzyme, was incubated at 30 °C with substrate, and test compounds in the presence of 100 uM ATP/ [gamma-32P] ATP. 32P incorporation was measured with a Top Count (Packard). IC50 values were calculated from dose-response curves; from these calculated apparent inhibition constants (Ki) were obtained using the Cheng-Prusoff equation: Ki = IC50/ (1 + ([ATP]/Km)).
Affinity data for this assay
 

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Last update November 1, 2007
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