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| | TRPM8 receptor assay |
| Description: | In the first set of data, the potency and in vitro effects of test compounds were evaluated on cloned hTRPM8 channel (encoded by the human TRPM8 gene, expressed in CHO cells) using a Fluo-8 calcium kit and a Fluorescence Imaging Plate Reader (FLIPRTETRA ) instrument. The assays were conducted by ChanTest Corporation (Cleveland, Ohio 44128, USA). Test solutions were in a HEPES-buffered saline, in 384-well plates, and placed into the FLIPR instrument (Molecular Devices Corporation, Union City, Calif., USA). Four 4 to 8 concentrations were tested, with L-menthol as the positive control. The test cells were Chinese Hamster Ovary (CHO) cells stably transfected with human TRPM8 cDNAs. Concentration-response data were analyzed via FLIPR Control software) and fitted to a Hill equation for the EC50. The 95% Confidence Interval was obtained using GraphPad Prism 6 software. |
| Affinity data for this assay | |
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