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Assay Method Information |
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| | Inhibition Assay |
| Description: | Staphylococcus aureus: Fluorescence-detected RNA polymerase assays with S. aureus RNA polymerase were performed as in Example 2.1, using reaction mixtures containing (20uL): 0-100 nM test salt, 75 nM S. aureus RNA polymerase core enzyme, 300 nM S. aureus σ, 20nM 384 bp DNA fragment containing the bacteriophage T4 N25 promoter, 100uM ATP, 100uM GTP, 100uM UTP, 100uM CTP, 40 mM Tris-HCl, pH 8.0, 80mM NaCl, 5mM MgCl2, 2.5mM DTT, and 12.7% glycerol. |
| Affinity data for this assay | |
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