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Assay Method Information

Assay Name:  Protease Inhibition Assay
Description:  Assay of HIV protease inhibition was performed by peptide cleavage using the substrate Val-Ser-Gln-Asn-beta-naphthylalanine*Pro-Ile-Val. Products of the cleavage reaction were quantified by HPLC detection, and data were fit to nonlinear least squares equations for determination of steady-state Michaelis-Menten kinetics.
Affinity data for this assay
 

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Last update November 1, 2007
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