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| | Inhibition of RET Family Kinases Activity |
| Description: | The activity assay method for the above kinases was established by homogeneous time-resolved fluorescence (HTRF), and the inhibitory activity of the compounds was determined. An 8 μL reaction solution was prepared, comprising 1×enzymatic buffer (Cisbio, HTRF KinEASE-TK), 5 mM MgCl2, 1 mM MnCl2, 1 mM DTT, 1 μM TK substrate-biotin (Cisbio, HTRF KinEASE-TK), 10 μM ATP (1 μM for RET WT, CCDC6-RET; 20 μM for KDR), gradient concentrations of compounds and the following concentrations of related kinases: 0.03 ng/μL RET WT, 0.2 ng/μL RET (V804M), 0.04 ng/μL RET (M918T), 0.12 ng/μL CCDC6-RET and 0.02 ng/μL KDR. The kinases and compounds were pre-incubated for 5 min, and then ATP and substrate were added to start the reaction. All the enzyme-catalyzed reactions were carried out at 25° C. for 60 min. After the enzyme-catalyzed reactions were completed, 4 μL of TK antibody-cryptate and 4 μL of streptavidin-XL665 (the reaction concentration was 62.5 nM) were added to the reaction mixtures, and the mixtures were incubated at 25° C. for another 60 min. After the incubation was completed, the HTRF fluorescence values were determined on CLARIOstar (BMG LABTECH), and IC50 was calculated using the GraphPad Prism 5.0 software. |
| Affinity data for this assay | |
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