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Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  The cholinesterase assays were performed using colorimetric method reported by Ellman. Enzyme activity was determined by measuring the absorbance at 405 nm. The activities were compared to the activity of a drug-free control and expressed as a percent of activity. IC50 values were determined at 0.2 mM total substrate concentration from a plot of percent enzyme activity versus -log[I], where [I] refers to inhibitor concentration. The presented values are the averages of three determinations.
Affinity data for this assay
 

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Last update November 1, 2007
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