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Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  The cholinesterase assays were performed using colorimetric method reported by Ellman. Enzyme activity was determined by measuring the absorbance at 405 nM. Determinations of IC50 were made by least-squares fitting of the Hill equation to the percentage inhibition data. The presented values are the average of at least four determinations. Standard errors are less than 30% of the mean IC50 values.
Affinity data for this assay
 

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Last update November 1, 2007
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