Assay Method Information |
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| Kinase Inhibition Assay |
Description: | In vitro kinase assay using purified enzyme, was incubated at room temperature with substrate, and test compounds in the presence of 100 uM ATP/ [gamma-33P] ATP. 33P incorporation was measured with scintillation counter. IC50 values were calculated from dose-response curves; from these calculated apparent inhibition constants (Ki) were obtained using the Cheng-Prusoff equation: Ki = IC50/ (1 + ([ATP]/Km)). |
Affinity data for this assay | |
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