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Assay Method Information

Assay Name:  ChC Enzyme Inhibition Assay
Description:  The rate of hydrolysis was determined from the change in absorbance at 324 nm using an extinction coefficient, 24700 M-1 cm-1 for FALGPA. Initial velocities were thus estimated using the direct linear plot-based procedure. The inhibition constant Ki was then determined by using Easson-Stedman plots and a linear regression program.
Affinity data for this assay
 

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Last update November 1, 2007
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