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| | In Vitro Binding for Human Carbonic Anhydrase II |
| Description: | The binding of test compounds to purified human erythrocyte carbonic anhydrase II was determined by a fluorescence competition assay using a Perkin-Elmer MPF-44B fluorescence spectrophotometer. The excitation and emission wavelengths were set at 280 and 460 nm, respectively. Fluorescence intensities were recorded, corrected for dilution by the titrant, and fitted by nonlinear least squares to a model in which the compound and dansylamide compete for a single binding site. All binding determinations were done a minimum of three times. |
| Affinity data for this assay | |
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