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Assay Method Information

Assay Name:  Kinase SPA Assay
Description:  The biochemical activity of compounds was determined by incubation with specific enzymes and substrates in the presence ATP/[gamma-33P] ATP. After incubation, the reaction was stopped and the phosphorylated substrate was separated from nonincorporated radioactive ATP using SPA beads (Amersham Pharmacia Biotech), counted using TopCount (Packard) to measure substrate-incorporated phosphate.
Affinity data for this assay
 

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Last update November 1, 2007
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