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Assay Method Information |
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| | AlphaScreen Assay |
| Description: | Experiments were performed in white opaque 384-well plates from PerkinElmer (Waltham, Mass.), and the samples were read on a Synergy 2 plate reader (Biotek, Winooski, Vt.) with a sensitivity setting of 200 using AlphaScreen protocol with excitation at 680 nm and emission at 570 nm. All dilutions were made in 1× assay buffer containing 25 mM HEPES (pH 7.4), 100 mMNaCl and 0.1% BSA to minimize nonspecific interactions. For inhibitor competition experiment, 5 nM of C-terminal biotinylated Tcf4 45-mer, 20 nM of N-terminal His6-tagged β-catenin and inhibitors at different concentration were incubated in 20 μL of assay buffer for 2 h. Donor and acceptor beads were added to a final concentration of 10 μg/mL in 25 μL of assay buffer. The mixture was incubated at room temperature for 1 h. For each inhibitor competition assay, the negative controls (equivalent to 0% inhibition) refer to 5.0 nM of biotinylated Tcf4 45-mer, 20 nM of β-catenin, 10 g/mL donor and acceptor beads, but no tested peptide inhibitor was presented. The positive controls (equivalent to 100% inhibition) refer to only 5.0 nM of biotinylated Tcf4 45-mer and 10 g/mL donor and acceptor beads in a final volume of 25 μL. The IC50 value was determined by nonlinear least-square analysis of GraphPad Prism 5.0. Experiments were performed in triplicate and carried out in the presence of 1% DMSO. |
| Affinity data for this assay | |
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