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Assay Method Information |
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| | Liquid growth assay |
| Description: | Saturated ACT1 wild type (WT) as well as engineered ACT1 R183K (R183K) and ACT1 R335K (R335K) cultures were adjusted to 10 OD600 and 3 uL of serial 1:3 dilutions spotted on YPD agar supplemented with indicated concentrations of Chivosazole F. Growth was scored after 3 days incubation at 30 °C. |
| Affinity data for this assay | |
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