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| | DHODH Inhibition Assay |
| Description: | The assays were carried out by using a colorimetric DCIP method, which uses the colorimetric reagent 2, 6-dichlorophenolindophenol as the final electron acceptor. DCIP reduction is stoichiometrically equivalent to oxidation of dihydroorotate. Changes in absorbance were quantified in triplicate on a plate reader and data were analyzed to measure the reduction of DCIP as a decrease in absorbance at 600 nm. The data were then processed with Graphpad Prism to determine the IC50 and the apparent enzyme inhibition constant (Ki) for each inhibitor. Ki values were calculated using the Cheng-Prusoff equation with the Km for CoQD of 13.4 uM and 14.0 uM for PfDHODH and HsDHODH respectively. |
| Affinity data for this assay | |
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