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Assay Method Information |
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| | BACE-1 Inhibition Assay |
| Description: | Activity of BACE-1 was measured by monitoring the cleavage of its peptide substrate on a Fluoroskan Ascent plate reader with excitation and emission wavelengths of 355 and 530 nm, respectively. Initial reaction rates were measured, and IC50s were calculated from replicate curves using GraphPad Prizm software. |
| Affinity data for this assay | |
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