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Assay Method Information |
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| | MMP Assay |
| Description: | MMP-13 was assayed its proteolytic activity using a quenched fluorogenic substrate. The substrate hydrolysis was monitored by recording the increase in fluorescence. IC50 was determined using the formula: Vi/V0 = 1/(1 + [I]/ IC50), where Vi is the initial velocity of substrate cleavage in the presence of the inhibitor at concentration [I] and V0 is the initial velocity in the absence of the inhibitor. |
| Affinity data for this assay | |
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