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| | Ramos Cell Adhesion Assay (alpha4beta1 Mediated Adhesion/VCAM-1) |
| Description: | Immulon 96-well plates were coated with recombinant hVCAM-1 overnight at 4 C. Plates were washed and blocked for 1 h at room temperature in PBS-BSA. The compounds to be tested were added. Ramos cells, labeled with Calcein AM (Molecular Probes), were added to each well and allowed to adhere for 1 h at room temperature. Plates were washed, and cells were lysed. The plate was read at 485 nm excitation and 530 nm emission. |
| Affinity data for this assay | |
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