Assay Method Information |
|
| Kinase Assay |
Description: | The inhibitory effect of the compounds of the present invention on the activity of c-Met was confirmed as follows.Specifically, 250 μM G4Y1 peptide, which serves as a substrate for c-Met enzyme (2 ng/μL), and 50 μM ATP were subjected to an enzyme reaction in a reaction buffer (40 mM Tris-HCl (pH 7.5), 20 mM MgCl2, 0.1 mg/mL bovine serum albumin, and 50 μM DTT). The compounds prepared in Examples and comparative compounds were treated at various concentrations and reacted at room temperature for 1 hour, sequentially added with ADP-Glo Reagent and the Kinase detection reagent, and reacted at room temperature for 40 minutes and 30 minutes, respectively. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |