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Assay Method Information |
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| | Alpha Screen competitive assay |
| Description: | Assays were performed as described previously with minor modifications(Philpott et al., 2011). All reagents were diluted in 25 mM HEPES, 100 mM NaCl, 0.1 % BSA, pH 7.4 supplemented with 0.05 % CHAPS and allowed to equilibrate to room temperature prior to addition to plates. |
| Affinity data for this assay | |
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