Assay Method Information |
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| IMPDH2 Enzyme Inhibition and Human T-Lymphoblast (CEM) Proliferation Inhibition Assays |
Description: | Inhibition assays were performed using recombinant human IMPDH type 2 and varying concentrations of inhibitor. The production of NADH was monitored by following changes in absorbance at 340 nm. The concentrations of compound required to inhibit NADH accumulation by 50% (IC50) were calculated using a four-parameter logistic plot. The in vivo activities of compounds were also evaluated the inhibition of T-cell proliferation in CEM lymphoblast cells. The EC50/IC50 values were calculated using a four-parameter logistic plot. |
Affinity data for this assay | |
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