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Assay Method Information

Assay Name:  Estrogen Receptor Binding Assay and Ishikawa Assay
Description:  The binding affinities were determined by a competitive radiometric binding assay using [3H]estradiol as tracer. The Kd for 3H-estradiol was determined by saturation binding to ER receptors. The IC50 values for compounds were converted to Ki using Cheng-Prusoff equation. Estrogenic stimulation and antagonism were measured in Ishikawa human endometrial tumor cells by alkaline phosphatase quantitation. The data were fitted to a linear interpolation to derive IC50 values for the antagonist mode and a percentage efficacy was calculated that blocks the 17beta-estradiol (1nM) stimulus.
Affinity data for this assay
 

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Last update November 1, 2007
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