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| | Papain Inhibition Assay |
| Description: | Enzyme activities were calculated from kinetic measurements performed by spectrophotometric detection of the product p-nitroaniline (pNA) at wavelength of 405 nm. Progress curves were monitored over 10 min. Rate was determined for 7 different inhibitor concentrations in duplicate. The apparent inhibition constant, Ki (app) was determined by fitting equation to the experimental data. The true inhibition constant, Ki values were calculated by correction of Ki (app) according to the equation: Ki = Ki(app) /(1 + [S]/Km). |
| Affinity data for this assay | |
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