Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | FLIPR (Fluorescence Imaging Plate Reader)-Based Ca2+ Assay |
| Description: | One day before the assay was performed, human TRPV1 expressed in 1321N1 astrocytoma cells were plated onto 96-well assay plates and grown until 2.5 h before the assay, and the cells were loaded with the Ca2+ reporter dye Fluo3-AM for 2 h at room temperature. After this, the cells were washed, and continuously being incubated for 30 min with medium containing test compounds at a range of different concentrations. The plates were then placed into a FLIPR to monitor fluorescence (excitation @ 488 nm and emission @540 nm) before and after the addition of capsaicin, antagonist potency was determined versus an EC80 concentration of capsaicin that was determined empirically on the day of the experiment. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||