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Assay Method Information

Assay Name:  HMG-CoA Reductase Enzyme Assay and Inhibition of Cellular Cholesterol Synthesis Assay
Description:  Enzyme Assay for HMG-CoA reductase was based on the conversion of isotopically labeled HMG-CoA to mevalonic acid using rat liver microsomes as enzyme source. The product, [14C]-mevalonic acid was lactonized and separated from excess substrate via an anion ion exchange resin column, and radioactivity was counted on a scintillation counter. The IC50 values were calculated with GraphPad software (Prism). The cellular assay EC50/IC50 values were obtained from inhibition of cholesterol synthesis in rat hepatocyte.
Affinity data for this assay
 

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Last update November 1, 2007
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