Assay Method Information |
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| Fluorescence Imaging Plate Reader (FLIPR) Assay |
Description: | The functional FLIPR assay was developed from H4 glioma cells in which expression of the endogenous human GHS receptor was enhanced by RAGE-activation. The assay utilized the GHS receptor natural signaling through a calcium phospholipase C mediated pathway; agonists could be tested for their ability to stimulate intracellular calcium mobilization using a calcium-sensitive fluorescent probe. The EC50 was measured by determining the intracellular calcium concentration in a FLIPR assay, with ghrelin serving as the full agonist reference standard. All of the compounds were determined to be full agonist of the receptor. |
Affinity data for this assay | |
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