Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Enzyme Inhibition Assay |
| Description: | Enzyme activity was assayed in a 96-well plate format. TbcatB was incubated with inhibitor compound at room temperature for 20 min before the addition of substrate. V0 was determined by monitoring fluorescence (ex: 350 nm, em: 460 nm) on an Envision plate reader (Perkin-Elmer) for 10 min. All data were collected in triplicate. IC50 values were determined by sigmoidal curve-fitting by GraphPad Prism software. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||