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| | Radioligand Binding Assay |
| Description: | The assay was initiated by the addition of membranes to binding buffer containing test compound, blocker, and radioligand. The samples were incubated in triplicate. Brandel cell harvesters were used to filter the samples over Whatman GF/B filters, which were presoaked in wash buffer. The 125I retained on the filters was measured in a gamma counter. |
| Affinity data for this assay | |
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